uvrA [2020-09-29 09:55:46]

excinuclease ABC (subunit A), required for transcription-dependent asymmetry in mutation rates of genes in the two orientations

locus: BSU_35160

pI: 5.84

mw: 105.83 kDa

protein length: 957 aa

gene length: 2874 bp

function: DNA repair after UV damage

product: excinuclease ABC (subunit A)

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins]

Gene

Coordinates: 3,610,064 3,612,937

Phenotypes of a mutant

• reduced stationary phase mutagenesis [Pubmed|27399782]
• a [gene|39B73AADBFD42203ADFA2E834697799E6430A086|uvrA] [gene|DEFBDC2AB8E43FB06C65389160E8EACFE5FB9705|uvrB] mutant is sensitive to blue light-induced DNA damage [pubmed|30054368]
• sensitive to Cr(VI) treatment [pubmed|30745368]
• reduced resistance towards electron beams [pubmed|31948638]

The protein

Protein family

• [SW|ABC transporter superfamily] (according to UniProt)

[SW|Domains]

• 2 [SW|ABC transporter domain]s (aa 309-587, aa 607-935) (according to UniProt)

Structure

• [PDB|2R6F] (Geobacillus stearothermophilus)

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|DEFBDC2AB8E43FB06C65389160E8EACFE5FB9705|uvrB]-[gene|39B73AADBFD42203ADFA2E834697799E6430A086|uvrA]
  description: [Pubmed|22383849]

  regulatory mechanism

  • [protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA]: repression, [Pubmed|8226626], in [regulon|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA regulon]

  regulation

  • induced upon DNA damage ([protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA]) [Pubmed|8226626]

  additional information

  • during [protein|search|sporulation] expressed both in the forespore and the mother cell [PubMed|24118570]
  • expression is induced in the presence of Cr(VI) [pubmed|30745368]

Biological materials

Mutant

• GP3534 (Δ([gene|39B73AADBFD42203ADFA2E834697799E6430A086|uvrA]-[gene|DEFBDC2AB8E43FB06C65389160E8EACFE5FB9705|uvrB])::kan), available in [SW|Jörg Stülke]'s lab
• GP1175 (Δ([gene|39B73AADBFD42203ADFA2E834697799E6430A086|uvrA]-[gene|DEFBDC2AB8E43FB06C65389160E8EACFE5FB9705|uvrB])::ermC), available in [SW|Jörg Stülke]'s lab [pubmed|22178973]
• BKE35160 ([gene|39B73AADBFD42203ADFA2E834697799E6430A086|uvrA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE35160 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TATCCGATCCATAGCCATTT, downstream forward: _UP4_TAAAACCCTCTGTTAAGAGG
• BKK35160 ([gene|39B73AADBFD42203ADFA2E834697799E6430A086|uvrA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK35160 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TATCCGATCCATAGCCATTT, downstream forward: _UP4_TAAAACCCTCTGTTAAGAGG

References

Reviews

• 16464004,15927210,7801120,22933559

Original publications

• 11751826,21145481,8226626,24118570,24118570,25713353,27399782,30054368,30745368,31948638